# decoupler

**URL:** https://discourse.scverse.org/c/help/decoupler/47.md

[Latest](https://discourse.scverse.org/latest.md) · [Categories](https://discourse.scverse.org/categories.md) · [Tags](https://discourse.scverse.org/tags.md)

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## [About the decoupler category](https://discourse.scverse.org/t/about-the-decoupler-category/3835)

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**Author:** [@Zethson](https://discourse.scverse.org/u/Zethson)\
**Replies:** 0

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## [Aggregating ULM cell-type scores by cluster](https://discourse.scverse.org/t/aggregating-ulm-cell-type-scores-by-cluster/4044)

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**Author:** [@albi](https://discourse.scverse.org/u/albi)\
**Replies:** 1\
**Last updated:** [September 7, 2026, 11:48pm UTC](https://discourse.scverse.org/t/aggregating-ulm-cell-type-scores-by-cluster/4044 "2026-09-07T23:48:35Z")

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Hi! I am using decoupler.mt.ulm with a network of cell-type marker genes to obtain cell-type enrichment scores, basically following the cell-type scoring approach shown in the single-cell enrichment analysis tutorial. I…

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## [Implementation of PROGENy pathway analysis](https://discourse.scverse.org/t/implementation-of-progeny-pathway-analysis/3972)

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**Author:** [@zanebeckwith](https://discourse.scverse.org/u/zanebeckwith)\
**Replies:** 2\
**Last updated:** [May 1, 2026, 8:19pm UTC](https://discourse.scverse.org/t/implementation-of-progeny-pathway-analysis/3972 "2026-05-01T20:19:47Z")

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Sorry if this is a naive question, but I’m trying to check my understanding of the PROGENy approach to pathway analysis, and scverse/decoupleR’s implementation of it. In the original PROGENy paper, I see that the PROGEN…

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## [Interpreting decoupler results](https://discourse.scverse.org/t/interpreting-decoupler-results/3962)

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**Author:** [@jpagolia](https://discourse.scverse.org/u/jpagolia)\
**Replies:** 1\
**Last updated:** [April 16, 2026, 5:27pm UTC](https://discourse.scverse.org/t/interpreting-decoupler-results/3962 "2026-04-16T17:27:45Z")

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I am using decoupler’s univariate linear model approach to infer transcription factor activity based on Collectri GRNs. When I run decoupler.rankby\_group() with the Wilcoxon test to obtain a list of the top inferred tran…

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## [Filter\_by\_expr parameters Decoupler](https://discourse.scverse.org/t/filter-by-expr-parameters-decoupler/3955)

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**Author:** [@npont](https://discourse.scverse.org/u/npont)\
**Replies:** 4\
**Last updated:** [April 8, 2026, 2:49pm UTC](https://discourse.scverse.org/t/filter-by-expr-parameters-decoupler/3955 "2026-04-08T14:49:40Z")

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Hello, I am struggling to understand the filtering done in decoupler.pp.filter\_by\_expr. I first used the pl.filter\_by\_expr to visualize how genes are expressed in my pseudobulk samples using: dc.pl.filter\_by\_expr( ad…

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## [Impact of tmin parameter (decoupler.mt.ulm)](https://discourse.scverse.org/t/impact-of-tmin-parameter-decoupler-mt-ulm/3947)

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**Author:** [@npont](https://discourse.scverse.org/u/npont)\
**Replies:** 1\
**Last updated:** [April 6, 2026, 6:06pm UTC](https://discourse.scverse.org/t/impact-of-tmin-parameter-decoupler-mt-ulm/3947 "2026-04-06T18:06:25Z")

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Hello, I wonder the impact of modifying tmin parameter in decoupler.mt.ulm when focusing on Gene Regulatory Networks . As a recall, tmin is the minimum number of targets per source. I guess by increasing tmin we have l…

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## [Multi-threading support in decoupler](https://discourse.scverse.org/t/multi-threading-support-in-decoupler/3859)

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**Author:** [@amit491](https://discourse.scverse.org/u/amit491)\
**Replies:** 1\
**Last updated:** [April 6, 2026, 6:01pm UTC](https://discourse.scverse.org/t/multi-threading-support-in-decoupler/3859 "2026-04-06T18:01:54Z")

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Hello, This is my first post in the forum here. I am trying to implement Transcription factor (TF) activity estimation on large scRNA dataset. I tried the R implementation of the decoupler package, but the run\_ulm step…