# Comparing DEGs within same cluster in lineage traced mice

**URL:** <https://discourse.scverse.org/t/comparing-degs-within-same-cluster-in-lineage-traced-mice/1359>\
**Category:** General\
**Created:** [April 27, 2023, 6:45pm UTC](https://discourse.scverse.org/t/comparing-degs-within-same-cluster-in-lineage-traced-mice/1359 "2023-04-27T18:45:43Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![ReneVQ](https://yyz1.discourse-cdn.com/flex035/user_avatar/discourse.scverse.org/renevq/32/602_2.png) [@ReneVQ](https://discourse.scverse.org/u/ReneVQ)\
**Post date:** [April 27, 2023, 6:45pm UTC](https://discourse.scverse.org/t/comparing-degs-within-same-cluster-in-lineage-traced-mice/1359/1 "2023-04-27T18:45:43Z")

</div>

Hi,

I’m still pretty new to bioinformatics, and still have a lot to learn. We’ve been working on small molecule drugs for cell regeneration, and are using TdTomato (TdT) labeled mice for lineage tracing. We can detect TdT+ cells in our end-state cluster, and want to compare DEGs between the TdT+ and TdT- cells to see how close/far we are from getting mature cells. How would I go about segregating the cells by TdT expression so I can run sc.tl.rank\_genes\_groups?

Thanks,  
René VQ