Best Practices for Multi-Panel Figures

Hello everyone,

I am putting together a multi-panel figure containing bioinformatic plots from that are getting output from a variety of different python scRNAseq packages. One problem I am encountering is that when I use different plotting functions, or variable types - the plot aspect ratio doesn’t necessarily remain the same even though I have the same figsize. For example, a UMAP of the same data will be wider or thinner depending on whether the color variable is continuous or categorical, due to the legend width vs colorbar width. Is there any way to ensure that the scatter plot (Axes object) remains the same aspect ratio and size? One way I have considered is selecting just the plot/points in adobe illustrator , and resizing so they are the same in both plots. Is this considered acceptable? Really appreciate any advice you all can give me!

Thanks!