How I can use big data with scanpy?

Help scanpy
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Hi,
Thanks for developing scanpy.
I want to run scanpy on kaggle or Colab but they have 30gb and 12gb of RAM, respectively. When I read my data with “read_h5ad()”, Colab is crash and 25gb of Keggle’s RAM is occupied and when I want use “sc.tl.rank_group_genes()”, Kaggle is crashed. When I want to read data by “backed = ‘r’ or ‘r+’”, “sc.tl.rank_group_genes()” give me error. Can you help me How I can load this data withoud crash in Colab?
thanks.

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Here is the link of one of the notebook for my students… Feel free to use it if helps.
https://www.kaggle.com/code/babumiaphd/ibe-may-13th-tutorial-scanpy