Hello,
I wonder the impact of modifying tmin parameter in decoupler.mt.ulm when focusing on Gene Regulatory Networks .
As a recall, tmin is the minimum number of targets per source. I guess by increasing tmin we have less TF in the analysis meaning less multiple comparisons and thus lower false positive rate (= TF reported to be enriched but are not). While by decreasing tmin, we also include TF with just a few targets and therefore offer allow new discoveries (better for exploration) at the cost of increasing the noise and false positive rate. Is it correct ?
Thank you for any insight