DestVI gene imputation comparison between slides


thank you for your great software. We used DestVI to calculate cell proportion values and impute gene expression values in two different tissues and on multiple slides. We are very satisfied with the cell proportion estimates (based on comparison with the tissue histology) and with the gene imputation values (based on biological insights).

The questions we have are:

Are the imputed gene expression values for each spot and cell type comparable between slides of the same tissue (same scRNA-seq reference used for training)?

Are they comparable between different tissues (different scRNA-seq reference used for deconvolution)?

Thank you very much for your help.