Cell assign +/- marker expression possible? like 0.5?

say i have 3 cell types with 5 markers

and marker number 5 which really helps distinguish type A and B, is Sometimes sometimes not in type C

can I make a marker that instead of 1 is 0.5? the two example sheets (HGSC & FL) are only binary 0 and 1s , so I am not sure if this is possible or would i have to create separate types or a + and - type grouping?


Not exactly, although if a gene is a marker of a cell type, it has to induce a certain positive log fold change of expression over a baseline. It could be interesting to modify the code to allow marker specific thresholds there; but this is beyond the scope of the original method (for which I am not an author and we only provide a reimplementation here).